Prof. Dr. Stefan Johannes Wittke

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  Arbeits- und Forschungschwerpunkte
  
  Elementspeziesanalytik in marinen und terrestrischen Organismen
  Stressinduzierte Wachstumsbeeinträchtigungen bei marinen Organismen
  biopharmazeutische Grundlagenforschung
  
  Qualitätssicherung in der Biopharmazie
  
  
  Publikationen:
  
  Jahn, H., Wittke, S., Zürbig P., Raedler T.J., Arlt S., Kellmann M., Mullen W., Eichenlaub M., Mischak H., Wiedemann K.; Peptide fingerprinting of Alzheimer´s disease in cerebrospinal fluid: Identification and prospective evaluation of new synaptic biomarkers, PLoS, 2011 (ahead of print)
  
  May M, Brookman-May S, Hoschke B, Gilfrich C, Kendel F, Baxmann S, Wittke S, Kiessig ST, Miller K, Johannsen M; Ten-year survival analysis for renal carcinoma patients treated with an autologous tumour lysate vaccine in an adjuvant setting, Cancer Immunol Immunother. 2010 May;59(5):687-95. Epub 2009 Oct 30
  
  Decramer S, Zuerbig P, Wittke S, Mischak H, Bascands JL, Schanstra JP. Identification of urinary biomarkers by proteomics in newborns: use in obstructive nephropathy. Contrib Nephrol. 2008;160:127-41
  Raedler TJ, Wittke S, Jahn H, Koessler A, Mischak H, Wiedemann K Capillary electrophoresis mass spectrometry as a potential tool to detect lithium-induced nephropathy: Preliminary results. Prog Neuropsychopharmacol Biol Psychiatry. 2008 Apr 1;32(3):673-8
  
  Julian BA, Wittke S, Haubitz M, Zürbig P, Schiffer E, McGuire BM, Wyatt RJ, Novak J. Urinary biomarkers of IgA nephropathy and other IgA-associated renal diseases. World J Urol. 2007 Oct;25(5):467-76.
  Julian BA, Wittke S, Novak J, Good DM, Coon JJ, Kellmann M, Zürbig P, Schiffer E, Haubitz M, Moldoveanu Z, Calcatera SM, Wyatt RJ, Sýkora J, Sládková E, Hes O, Mischak H, McGuire BM.Electrophoretic methods for analysis of urinary polypeptides in IgA-associated renal diseases. Electrophoresis. 2007 Dec;28(23):4469-83.
  
  S. Wittke, E. Schiffer, H. W. Bauer. Kapillarelektrophorese gekoppelte Massenspektrometrie zur Proteomanalyse als innovative diagnostische Methode bei Prostata- und Blasenkrebs. Der Urologe, (2007)
  Zürbig P, Renfrow MB, Schiffer E, Novak J, Walden M, Wittke S, Just I, Pelzing M, Neusüss C, Theodorescu D, Root KE, Ross MM, Mischak H. Biomarker discovery by CE-MS enables sequence analysis via MS/MS with platform-independent separation. Electrophoresis. 2006 Jun;27(11):2111-25
  D. Theodorescu, S. Wittke, M.M. Ross, M. Walden, M Conaway, I. Just, H. Mischak, H. F. Frierson: Discovery and validation of new protein biomarkers for urothelial cancer: a prospective analysis. Lancet Oncology, 2006, 7: 230-40.
  Stephane Decramer, Stefan Wittke, Harald Mischak, Petra Zürbig, Michael Walden, François Bouissou, Jean-Loup Bascands and Joost P Schanstra. Predicting the clinical outcome of congenital unilateral ureteropelvic junction obstruction in newborn by urinary proteome analysis. Nature Med. 2006, 12(4):398-400.
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  S. Wittke, M. Haubitz, M. Walden, F. Rohde, A. Schwarz, M. Mengel, H. Mischak, W. Gwinner, Detection of acute rejection by proteomic analysis of urinary samples in renal transplant recipients, Am. J Transpl. (2005).
  M. Haubitz, S. Wittke, E. M. Weissinger, M. Walden, H. D. Rupprecht, J. Floege, H. Haller, H. Mischak, Urine protein patterns can serve as diagnostic tools in patients with IgA nephropathy, Kidney Int. (2005), 67, 2313-20.
  D. Theodorescu, D. Fliser, S. Wittke, H. Mischak, R. Krebs, M. Walden, A. Semjonow Capillary electrophoresis coupled to mass spectrometry as a tool to define prostate cancer biomarkers: a preliminary study, Electrophoresis, (2005), 26, 2797-2808.
  M. J. Chalmers, C.L. Mackay, C.L. Hendrickson, S. Wittke, M. Walden, H. Mischak, I. Just, A.G. Marshall, Biomarkers for End Stage Renal Diseases: A Combined Top-Down and Bottom-Up Mass Spectrometric Approach to Characterization and Statistical Validation, Analytical chemistry, 2005, 15;77(22):7163-71.
  
  E.M. Weissinger, S. Wittke, T. Kaiser, H. Haller, S. Bartel, R. Krebs, I. Golovko, H.D. Rupprecht, M. Haubitz, H. Hecker, H. Mischak, D. Fliser, Proteomic patterns established with capillary electrophoresis and mass spectrometry for diagnostic purposes, Kidney Int. (2004), 65 (6), 2426ff.
  H. Mischak, T. Kaiser, Michael Walden, M. Hillmann, S. Wittke, A. Herrmann, S. Knueppel, H. Haller, D. Fliser, Proteomic analysis for the assessment of diabetic renal damage in humans, Clinical Science, (2004) 107, 485-95.
  N. v. Neuhoff, T. Kaiser, S. Wittke, R. Krebs, A. Pitt, A. Burchard, A. Sundmacher, B. Schlegelberger, W. Kolch, H. Mischak, Mass spectrometry for the detection of differentially expressed proteins: a comparison of surface-enhanced laser desorption/ionization and capillary electrophoresis/mass spectrometry, Rap. Com. Mass Spectrom. (2004), 18 (2), 149-56.
  
  S. Wittke, D. Fliser, M. Haubitz, S. Bartel, R. Krebs, F. Hausadel, M. Hillmann, I. Golovko, P. Koester, H. Haller, T. Kaiser, H. Mischak, E.M. Weissinger, Determination of peptides and proteins in human urine with capillary electrophoresis–mass spectrometry, a suitable tool for the establishment of new diagnostic markers, J Chrom. A, (2003), 1013 (1-2), 173-81.
  T. Kaiser, A. Hermann, J. T. Kielstein, S. Wittke, S. Bartel, R. Krebs, F. Hausadel, M. Hillmann, I. Golovko, P. Koester, H. Haller, E. M. Weissinger, D. Fliser, H. Mischak: Capillary electrophoresis coupled mass spectrometry to establish polypeptide patterns in dialysis fluids. J Chrom. A, (2003), 1013 (1-2), 157-73
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  Reviews
  
  S. Wittke, H. Mischak, W. Kolch, T. J. Raedler, K. Wiedemann; Discovery of biomarkers in human urine and cerebrospinal fluid by capillary electrophoresis coupled mass spectrometry: towards new diagnostic and therapeutic approaches. Electrophoresis (2005), 26, 1476-87.
  S. Wittke, T. Kaiser, H. Mischak, Differential Polypeptide display: The search for the elusive target, J. Chrom. B (2004), 803 (1), 17-26.
  T. Kaiser, S. Wittke, I. Just, R. Krebs, S. Bartel, D. Fliser, H. Mischak, E.M. Weissinger, Capillary Electrophoresis coupled mass spectrometry to establish polypeptide patterns in dialysis fluids, Electrophoresis, (2004) 25, 13, 2044-55.
  D. Fliser, S. Wittke, H. Mischak: Capillary electrophoresis coupled to mass spectrometry for clinical diagnostic purposes. Electrophoresis (2005), 26, 2708-16.

Vorträge;
DGPPN 2006: Berlin, Deutschland; The role of Proteomics in investigating psychiatric disorders: New methods, new possibilities? 25.11.2006
Medica 2006:Düsseldorf, Deutschland; Diagnose von Prostata- und Blasenkrebs durch nicht–invasive Urinuntersuchung, 18.11.2006
University of Alabama, Birmingham, Alabama, USA, Diagnosis or renal diseases using Capillary electrophoresis coupled mass spectrometry, 12.12.2005
University of Virginia, Charlottesville, Virginia, USA; Examination of human bodyfluids by Capillary electrophoresis coupled mass spectrometry for clinical diagnosis, 07.12.2005.
15th annual CE/MS conference Frederick, Maryland, USA, 17-19.Oct. 2004, S. Wittke, M. Walden, H. Mischak, Discovery of Biomarkers in Urine via CE-MS: towards new diagnostic and therapeutic approaches
Nephrologisches Seminar Uniklinik Tübingen, 21.11.2002, S. Wittke, Protein Profiling mittels Massenspektrometrie: Identifikation von Proteinmarkern in menschlichen Körperflüssigkeiten
Euroanalysis XI, 03.–09.09.2000, European Conference on Analytical Chemistry, Division of Analytical Chemistry, Federation of European Chemical Societies, FECS Event n° 246, Lissabon. S. Wittke, M. Grote, J. Brüggemann, Th. Betsche. Heavy metal binding compounds in seeds, leaves, stems and roots of sunflower and rape plants: a comparative investigation, Abstract Nr. OC 74.


Betreute Diplomarbeiten

S. Tiedt; Etablierung und Validierung der 1-dimensionalen-SDS-Polyacrylamid-Gelelektrophorese zur Analytik des Proteingehaltes und der Proteinzusammensetzung eines Tumorzelllysates (2009)
B. Raabe; PAK-Analytik mittels GC-MS und HPLC-FLD: Methodenentwicklung und Vergleich (2009)
S. Dyksma; „Kinetische Eigenschaften der Linalool Dehydratase-Isomerase (2010)
K. Gräfe; Extraktion und Aufbereitung von in Porenwasser gelösten Gasen aus Sedimenten zur Konzentrations- und Isotopenanalyse mittels Massenspektrometrie (2010)
T. Hirse; Optimierung einer Methode zur physikochemischen Kontrolle rezirkulierender Meerwasseraquarien (2011)
I. Kröger; Automatisierte Probenvorbereitung zur selektiven und hochempfindlichen Bestimmung von Vitamin B 2 , B 5 , B 6 und B 7 - Gehalten in Fischen (2011)
J. Mönnich; Funktionsanalyse mitochondrialer Komplexe antarktischer Fische am Beispiel von Lepidonotothen squamifrons (2011)
P. Schaal; Investigation of mussel shells (Mytilus edulis, Mytilus galloprovincialis) from natural and aquacultural sites: Differences in breaking load, Ca/Mg-ration and protein content (2011)


Angebote Bachelorarbeiten

 AS-Analytik.pdf
 Bachelorarbeit Etablierung Aminosäure-Analytik in Fischmehl

 Cholesterol-Analytik.pdf
 Bestimmung von Cholesterol in Fischen

 Lipid-Analytik.pdf
 Bestimmung und Quantifizierung von Lipiden in Fischmehlen